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KMID : 1177720180120010017
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Journal of Alternatives to Animal Experiments
2018 Volume.12 No. 1 p.17 ~ p.23
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Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance
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Park Sae-Ra
Cho Sun-A
Kim Young-So
An Su-Sun
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Abstract
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Although fragrance-free cosmetics are increasing in recent years, fragrance is still an important element of cosmetics. The phototoxicity of fragrance used in cosmetics is tested to confirm the safety before used. Because fragrances are mixtures of various aromatic compounds and exact composition of them are not disclosed by the fragrance company, it is difficult to analyze and/or presume the cause of phototoxicity. Therefore, it is important to use the test method having good applicability to various mixture and high reliability of results. In this study, we tried to establish an appropriate ITS approach to improve the reliability of the cosmetic fragrance toxicity test. Three animal alternatives, 3T3 neutral red uptake phototoxicity assay (OECD TG 432), Reactive oxygen species assay, Molar absorption (extinction) coefficient (MEC) measurement test were performed and coincidence rates among tests were compared. As a result, the application of the ITS approach wasn’t suitable for fragrances due to the lack of harmonization of the three test methods and different positive rates. 3T3 neutral red uptake phototoxicity assay presumed to be most suitable for the evaluation of the phototoxicity of fragrance in terms of safety. Reactive oxygen species assay and MEC measurement test were thought to be available to reconfirm the results of 3T3 neutral red uptake phototoxicity assay.
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KEYWORD
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Animal alternatives, 3T3 NRU PT assay, ROS assay, MEC, fragrance
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